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In BRAF V600Emutant melanoma, deep and single-cell sequencing reveals multiple resistant subpopulations before therapy that emerge in parallel under BRAF/MEK inhibition, explaining the frequent failure of sequential monotherapies

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In the standard 2D cell cultures, cells reveal a cobblestone shape, whereas in more advanced models with a dynamic flow, cells mimic the physiologic shape of vascular ECs in vivo, acquiring an elongated shape, due to shear stress [75]